Our topic is protein technology. There will be lectures and lab classes for PhD students and junior postdocs.

For MaxSynBio-Members and associated scientists only. Everyone else should register coordination office (Jakob Schweizer).

Monday, 15 May
from 19:00 onwards Social get-together at Hövels Hausbrauerei
Tuesday, 16 May
08:00 Registration
8:30-8:45 Philippe Bastiaens: Welcome Address
8:45-9:30 Peter Bieling: Protein Expression 1 incl. Biochemistry Basics
9:30-10:15 Peter Bieling: Protein Expression 2
10:15-10:30 Coffee break
10:30-11:15 Lei Kai: Cell-free Expression and Protein Characterization
11:15-12:00 Tim Bergbrede/Astrid Krämer: Dortmund Protein Facility
12:00-12:15 Break
12:15-13:15 Martin Engelhard: Chemical Protein Synthesis/Modification of Proteins
13:15-14:00 Lunch
14:00-18:00 Practicals (see below)
19:00-20:00 Dinner Buffet
20:00 Christin Kleineberg, Lado Otrin: Transmembrane Proteins
20:45 - 21:30 Protein Clinic 1*
Wednesday, 17 May
8:30-9:15 Lei Kai: Martinsried Protein Facility
9:15-10:00 Roger Goody: Biophysical Methods for analysis of protein interaction (kinetik + Thermodynamik)
10:00-10:15 Break
10:15-11:15 Konstantin Gavriljuk: Membrane Proteins/Compartments Methods
11:15-11:45 Ilia Platzman: Proteins & Compartments Methods
11:45-12:00 Break
12:00-13:15 Protein Clinic 2*
13:15-14:00 Lunch
14:00-18:00 Practicals (see below)
19:00 Dinner
19:00-21:00 Wrap-up & Feedback

*The sessions Protein Clinic 1 and 2 are intended as discussion space in which you can pose your questions regarding proteins to a panel of experts and inquire about the feasibility of specific approaches regarding your protein interest.

Practicals - Please sign up here:
1 Cell-Free Protein Expression (Lei Kai)
2 Optogenetic Systems (Solveig Bartelt, Elizaveta Chervyachkova)
3 Structural Methods by Pymol** (Jenny Keller, Marion Pesenti, Arsen Petrovic)
4 Purification of a Test Protein (Konstantin Gavriljuk)

Membrane Protein Interactions by TIRF  (Ilaria Visco)

6 Anisotropy to Measure Binding Affinity (Peter Bieling, Johanna Funk)

**Bring your own device! The practical course Structural Methods by Pymol is a computer-based course. If you want, you can bring your own laptop and we will install the program Pymol on your device. Please mark during the registration for the practical course if you want to bring your own laptop. We will provide computers for those who can't/don't want to bring their own device.

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