Program
Our topic is protein technology. There will be lectures and lab classes for PhD students and junior postdocs.
For MaxSynBio-Members and associated scientists only. Everyone else should register coordination office (Jakob Schweizer).
| Monday, 15 May | |
| Arrival | |
| from 19:00 onwards | Social get-together at Hövels Hausbrauerei |
| Tuesday, 16 May | |
| 08:00 | Registration |
| 8:30-8:45 | Philippe Bastiaens: Welcome Address |
| 8:45-9:30 | Peter Bieling: Protein Expression 1 incl. Biochemistry Basics |
| 9:30-10:15 | Peter Bieling: Protein Expression 2 |
| 10:15-10:30 | Coffee break |
| 10:30-11:15 | Lei Kai: Cell-free Expression and Protein Characterization |
| 11:15-12:00 | Tim Bergbrede/Astrid Krämer: Dortmund Protein Facility |
| 12:00-12:15 | Break |
| 12:15-13:15 | Martin Engelhard: Chemical Protein Synthesis/Modification of Proteins |
| 13:15-14:00 | Lunch |
| 14:00-18:00 | Practicals (see below) |
| 19:00-20:00 | Dinner Buffet |
| 20:00 | Christin Kleineberg, Lado Otrin: Transmembrane Proteins |
| 20:45 - 21:30 | Protein Clinic 1* |
| Wednesday, 17 May | |
| 8:30-9:15 | Lei Kai: Martinsried Protein Facility |
| 9:15-10:00 | Roger Goody: Biophysical Methods for analysis of protein interaction (kinetik + Thermodynamik) |
| 10:00-10:15 | Break |
| 10:15-11:15 | Konstantin Gavriljuk: Membrane Proteins/Compartments Methods |
| 11:15-11:45 | Ilia Platzman: Proteins & Compartments Methods |
| 11:45-12:00 | Break |
| 12:00-13:15 | Protein Clinic 2* |
| 13:15-14:00 | Lunch |
| 14:00-18:00 | Practicals (see below) |
| 19:00 | Dinner |
| 19:00-21:00 | Wrap-up & Feedback |
*The sessions Protein Clinic 1 and 2 are intended as discussion space in which you can pose your questions regarding proteins to a panel of experts and inquire about the feasibility of specific approaches regarding your protein interest.
| Practicals - Please sign up here: docs.google.com |
|
| 1 | Cell-Free Protein Expression (Lei Kai) |
| 2 | Optogenetic Systems (Solveig Bartelt, Elizaveta Chervyachkova) |
| 3 | Structural Methods by Pymol** (Jenny Keller, Marion Pesenti, Arsen Petrovic) |
| 4 | Purification of a Test Protein (Konstantin Gavriljuk) |
| 5 |
Membrane Protein Interactions by TIRF (Ilaria Visco) |
| 6 | Anisotropy to Measure Binding Affinity (Peter Bieling, Johanna Funk) |
**Bring your own device! The practical course Structural Methods by Pymol is a computer-based course. If you want, you can bring your own laptop and we will install the program Pymol on your device. Please mark during the registration for the practical course if you want to bring your own laptop. We will provide computers for those who can't/don't want to bring their own device.