#23 - GUV constriction and motion driven by MinDE Turing-like patterns

Meifang Fu (Max Planck Institute of Biochemistry, Martinsried)

Monday, 30 Nov 21:15 - 22:00 CET

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Abstract

Title: GUV constriction and motion driven by MinDE Turing-like patterns

Author(s): Meifang Fu^a, Petra Schwille^a

Affiliation(s): ^aMax Planck Institute of Biochemistry, Martinsried

Abstract: The E. coli MinDE system has become a paradigmatic reaction–diffusion system in biology. The membrane-bound ATPase MinD and ATPase-activating protein MinE oscillate between the cell poles, positioning the main division protein FtsZ at midcell. In vitro reconstruction of MinDE on supported lipid bilayer (SLB) results in Min travelling wave and Min stationary patterns that that closely resemble “Turing patterns”. Here, I put GUVs onto SLBs, and then initiating Min Turing-like pattern. Unexpectedly, the GUVs underwent repeated constriction and motion. To investigate the potential mechanism, buffer and protein concentrations were systematically changed. In this mini-talk, I will introduce the membrane system and the main phenomenal results, which hopefully can trigger further discussions.

References

[1] Glock, Philipp, Beatrice Ramm, Tamara Heermann, Simon Kretschmer, Jakob Schweizer, Jonas Mücksch, Gökberk Alagöz, and Petra Schwille. “Stationary Patterns in a Two-Protein Reaction-Diffusion System.” ACS Synthetic Biology 8, no. 1 (January 18, 2019): 148–57. https://doi.org/10.1021/acssynbio.8b00415.