#29 - Genetic context effects on gene expression from the E. coli chromosome
Scott Scholz (Max Planck Institute for Terrestrial Microbiology, Marburg)
Monday, 30 Nov 21:15 - 22:00 CET
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Title: Genetic context effects on gene expression from the E. coli chromosome
Author(s): Scott A. Scholza and Peter Freddolinob
Affiliation(s): aMax Planck Institute for Terrestrial Microbiology, Marburg, Germany; bUniversity of Michigan, Department of Biological Chemistry, Ann Arbor, USA
Abstract: To measure the effects of gene position on expression at high resolution across the bacterial genome, we built and analyzed a library of over 144,000 genome-integrated, standardized reporters in a single mixed population of Escherichia coli. By simultaneously tracking reporter barcodes from each integration site, we revealed previously undetected peaks of high transcriptional propensity centered on ribosomal RNA operons. Genes for amino acid biosynthesis were also specically enriched in highly transcribable regions, while prophages and mobile genetic elements were enriched in less transcribable regions, demonstrating that the E. coli chromosome has evolved gene-independent mechanisms for affecting expression from specific regions. Currently, we are combining features predicted to independently contribute to position-dependent expression variation to produce synthetic genomic contexts with extreme expression properties.