#40 - Creation of in-vitro actin cortices

#40 - Creation of in-vitro actin cortices

Kevin Kaub (Georg August University of Göttingen, Göttingen; Max Planck School Matter to Life)

Tuesday, 01 Dec 21:15 - 22:00 CET

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Title: Creation of in-vitro actin cortices

Author(s): Kevin Kauba,b, Peter Nietmanna, Niklas Wennera, Tabea Oswalda and Andreas Janshoffa,b

Affiliation(s): aGeorg August University of Göttingen, Göttingen, Germany; bMax Planck School Matter to Life

Abstract: To withstand extracellular stresses a mammalian cell is required to fine-tune its viscoelastic properties and as such the actin cortex, a main determinant for cellular mechanics, must be readily adjustable. The actin cortex is a thin protein meshwork consisting of actin and a plenitude of actin-related proteins, such as motor proteins (Myosin II), crosslinking proteins and polymerization-enhancing factors. Due to its complicated nature, a precise description of the interplay and viscoelastic influence of these different proteins remains elusive. In our work we focus to recreate such a cortical meshwork in a bottom-up approach by increasing the complexitiy of the system step-by-step. Furthermore, we review and present a number of different geometries in which the actin networks are confined in to study the impact of geometrical constraints on the microrheological properties. Lastly, we present a novel approach to study the emergence of actin structures within stabilized GUVs, which can be mass-produced using a microfluidic device [1].


[1] Weiss, Marian, Johannes Patrick Frohnmayer, Lucia Theresa Benk, Barbara Haller, Jan-Willi Janiesch, Thomas Heitkamp, Michael Börsch, et al. “Sequential Bottom-up Assembly of Mechanically Stabilized Synthetic Cells by Microfluidics.” Nature Materials 17, no. 1 (January 2018): 89–96. https://doi.org/10.1038/nmat5005.
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