#47 Programmable Liposome Fusion Mediated by Membrane Anchored DNA Oligomers

Philipp Löffler (University of Southern, Odense)

Monday, 30 Nov 21:15 - 22:00 CET

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Abstract

Title: Programmable Liposome Fusion Mediated by Membrane Anchored DNA Oligomers

Author(s): Philipp M. G. Löffler^a, Oliver Ries^a, Alexander Rabe^a, Nikolaj A. Risgaard^a and Stefan Vogel^a

Affiliation(s): ^aUniversity of Southern Denmark (Department of Physics, Chemistry and Pharmacy, Biomolecular Nanoscale Engineering Center)

Abstract: DNA-nanotechnology can address a demand in the field of synthetic biology for spatial and temporal control over nano- to microsized compartments. Our work uses Lipid-conjugated nucleic acid (LiNA) oligomers to functionalize liposomes in the 100 nm range. Any two liposomes that have complementary LiNAs on their surface can assemble and fuse via strand hybridization. In particular, membrane fusion leading to the mixing of liposome contents can be brought about when the LiNAs have a zipper-like design, i.e. with their anchors in close proximity in the duplex. Experiments with various molecular structures for anchoring and spacing have revealed that small changes of the structure near the lipid bilayer can have profound effects on fusion efficiency [1,2] and that the right LiNAs could be used for sequence-specific programming of multiple rounds of fusion [3]. The achieved level of control opens up possibilities for combinatorial or convergent syntheses in nanoreactors, compartmentalized enzymatic reactions as well as for a deeper understanding of membrane fusion processes.

References

[1] Ries, O., P. M. G. Löffler, A. Rabe, J. J. Malavan, und Stefan Vogel. „Efficient Liposome Fusion Mediated by Lipid–Nucleic Acid Conjugates“. Organic & Biomolecular Chemistry 15, Nr. 42 (31. Oktober 2017): 8936–45. https://doi.org/10.1039/C7OB01939D.

[2] Löffler, Philipp M. G., Anders Højgaard Hansen, Oliver Ries, Ulla Jakobsen, Alexander Rabe, Kristian T. Sørensen, Kasper Glud, und Stefan Vogel. „Lipidated Polyaza Crown Ethers as Membrane Anchors for DNA-Controlled Content Mixing between Liposomes“. Scientific Reports 9, Nr. 1 (25. September 2019): 13856. https://doi.org/10.1038/s41598-019-49862-y.

[3] Löffler, Philipp M. G., Oliver Ries, Alexander Rabe, Anders H. Okholm, Rasmus P. Thomsen, Jørgen Kjems, und Stefan Vogel. „A DNA-Programmed Liposome Fusion Cascade“. Angewandte Chemie International Edition 56, Nr. 43 (2017): 13228–31. https://doi.org/10.1002/anie.201703243.